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Immunohistochemistry of the histone trimethylation H3K27me3. Distinct differentiation of retained trimethylation with strong brown nuclear staining of all tumor cells (A) and loss of trimethylation with immunopositivity restricted to non-tumor cells like endothelium (B). Cases with partial staining of tumor cells were scored as retained (C) while samples with complete immunonegativity were excluded (D).
