FIG. 2.

Protection against calcium cytotoxicity. 661W cells seeded in 96-well plates were treated with calcium ionophore A23187 (final concentration 1 μM and 3% DMSO) to produce 50% cell death after 24 h (red traces). Seven compounds (CB1, 2, 3, 6, 10, 11, 12; ChemBridge, San Diego, CA) were added in concentrations of 0.1–10 μM (blue traces) and tested for efficacy using the MTT assay. Vehicle alone (green traces) or compounds alone (purple traces) had no effect on cell survival. Data represent mean per group and statistical significance (A23187 vs. drug+A23187, *P < 0.05) is indicated. DMSO, dimethyl sulfoxide; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.