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. Author manuscript; available in PMC: 2021 Aug 2.
Published in final edited form as: J Pathol. 2019 Nov 29;250(2):148–158. doi: 10.1002/path.5353

Figure 5.

Figure 5.

KRASG12V induces growth arrest in Fallopian tube epithelial cells. Short-term primary culture of Fallopian tube epithelial cells (Pt. X) and immortalised Fallopian tube cell line (FT2821) were transduced with KRASG12V under the control of a tetracycline-inducible promoter. (A) Cell growth was monitored by an ATP luminescence assay every 24 h, following treatment with indicated concentrations of doxycycline (n= 3). (B) Colony formation over 7 days of exposure to indicated concentrations of doxycycline (n= 3). (C) Immunoblot indicates increasing levels of ERK phosphorylation and p21 expression with increasing concentrations of doxycycline after 48 h of treatment. Graphs show mean ± SD, *p< 0.05, **p< 0.01, ***p< 0.001.