Figure 1 .

Bioinformatics analysis of scRNA-seq from the VZ and SVZ of the 16-18GW human fetal cortex. (a) 16p11.2 region and genes. (b) schematic adapted from Budday et al. 2015 shows the process of human fetal cortex development over time. Dotted box indicates approximately the time-period of interest for our study; 12-16PCW. (c) schematic of human cortical structure during development. (d) tSNE clustering of cell types. (e) changing mRNA expression levels of 16p11.2 genes as cells move from progenitors to neurons with KIF22 and ALDOA transcripts identified as changing significantly. Schematic of human cortex with dotted box indicates the compartments of the brain that were used to generate this dataset—the germinative VZ and SVZ. (f) Violin plots showing distribution of KIF22 in different cell types. (g) KIF22 gradient plot (arrow indicates progenitors with a subset expressing high levels of KIF22 mRNA). (h) Violin plots showing distribution of KIF22 at different cell cycle stages. (i) Violin plots showing distribution of ALDOA mRNA levels in different cell types. (j) ALDOA gradient plot. k) Violin plots showing distribution of ALDOA mRNA at different cell cycle stages.