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. Author manuscript; available in PMC: 2022 May 11.
Published in final edited form as: Methods Enzymol. 2021 May 11;656:459–494. doi: 10.1016/bs.mie.2021.04.010

Figure 6:

Figure 6:

Thioamide localization by HR-ESI-MS/MS in the M. acetivorans YcaO-TfuA reaction. (A) HR-ESI MS/MS analysis of the thioamidated product from the M. acetivorans YcaO/TfuA reaction with McrA 11-mer (GG-460RLGFFGFDLQD470, m/z 1444 Da). The b7 and b8 ions localize the thioamide modification to Gly465 (yellow circle). (B) HR-ESI MS of the thioamidated product. The error reported is calculated from the thioamide-containing product with the following molecular formula for the doubly-protonated ion: C66H95N17O18S. Adapted from Mahanta et al., 2018.