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. 2021 Jul 16;10:e66662. doi: 10.7554/eLife.66662

Key resources table.

Reagent type
(species)
or resource
Designation Source or
reference
Identifiers Additional
information
Recombinant DNA reagent pITy (plasmid) Parekh et al., 1996
Strain, strain background (Saccharomyces cerevisiae) BJ5464 Robinson Lab – Carnegie Mellon University
Strain, strain background (Escherichia coli) BL21 (DE3) Sigma, St. Louis, MO, USA #CMC0014
Chemical compound, drug DDM Anatrace, Maumee, OH, USA #D310
Chemical compound, drug CHAPS Anatrace, Maumee, OH, USA #C216
Chemical compound, drug CHS Anatrace, Maumee, OH, USA #CH210
Chemical compound, drug Xanthine amine congener Sigma, St. Louis, MO, USA #X103
Chemical compound, drug Theophylline Sigma, St. Louis, MO, USA #T1633
Commercial assay, kit Affigel 10 resin BioRad, Hercules, CA, USA #1536099
Commercial assay, kit Tricorn Superdex 200 10/300 GL column GE Healthcare, Pittsburgh, PA, USA #17-5175-01
Antibody Anti-A2AR, clone 7F6-G5-A2 (Mouse monoclonal) Millipore, Burlington, MA, USA #05-717 (1:500) dilution
Antibody Anti-Mouse IgG H&L DyLight 550 (Goat monoclonal) Abcam, Cambridge, MA, USA #ab96880 (1:600) dilution
Software, algorithm MODELLER 9.23 Eswar et al., 2006
Software, algorithm martinize.py script de Jong et al., 2013
Software, algorithm ELNeDyn elastic network Periole et al., 2009
Software, algorithm MARTINI coarse-grained force field v2.2 Monticelli et al., 2008
Software, algorithm GROMACS 2016 Abraham et al., 2015
Software, algorithm backward.py script Wassenaar et al., 2014
Software, algorithm LINCS Hess et al., 1997
Software, algorithm CHARMM36 and TIP3P force fields Best et al., 2012; Jorgensen et al., 1983
Software, algorithm LOOS Romo and Grossfield, 2009
Software, algorithm VMD Humphrey et al., 1996