Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Adgrg6 | GenBank | Gene ID: 215798 | |
| Genetic reagent (Mus musculus) | Adgrg6 conditional knockout | Taconic | Model #: TF0269 | Adgrg6f/f |
| Genetic reagent (Mus musculus) | B6.129S4-Gt(ROSA)26Sortm1Sor/J | Jackson Laboratory | Stock no.: 003474 | Rosa26-lsl-lacZ |
| Genetic reagent (Mus musculus) | ATC | Dy et al., 2012 | A gift from Dr. Véronique Lefebvre | |
| Genetic reagent (Mus musculus) | Col2a1-Cre | Long et al., 2001 | A gift from Dr. Fanxin Long | |
| Genetic reagent (Mus musculus) | Col2a1-CreERT2 | Chen et al., 2007 | A gift from Dr. Matthew Hilton | |
| Genetic reagent (Mus musculus) | Scx-Cre | Blitz et al., 2009 | A gift from Dr. Ronen Schweitzer | |
| Genetic reagent (Mus musculus) | B6.FVB-Tg(BGLAP-cre)1Clem/J | Jackson Laboratory | Stock no.: 019509 | Bglap-Cre |
| Genetic reagent (Mus musculus) | B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J | Jackson Laboratory | Stock no.: 006361 | Sp7-Cre |
| Cell line (Mus musculus) | ATDC5 | Sigma | Sigma: 99072806 | |
| Cell line (Mus musculus) |
Adgrg6 KO ATDC5 |
Liu et al., 2019 | CRISPR constructed to knock down the expression of Adgrg6 in ATDC5 cells | |
| Antibody | Anti-GPCR GPR126 (ADGRG6) (rabbit polyclonal) |
Abcam | ab117092 | IHC 1:500 |
| Antibody | Anti-SOX9 (rabbit Polyclonal) | EMD Millipore | AB5535 | IHC 1:200 |
| Antibody | Anti-collagen II Ab-2 (clone 2B1.5) (mouse monoclonal) | Thermo Scientific | MS235B | IHC 1:100 |
| Antibody | Recombinant anti-collagen I antibody (rabbit monoclonal) | Abcam | ab138492 | IHC 1:1000 |
| Antibody | Phospho-CREB (Ser133) (87G3) (rabbit monoclonal) | Cell Signaling | #9198 | IHC: 1:200 WB: 1:1000 |
| Antibody | GAPDH (14C10) | Cell Signaling | #2118 | WB: 1:2500 |
| Antibody | IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody | LI-COR | P/N: 926-32211 | WB: 1:10,000 |
| Sequence-based reagent | b-actin-F | Sigma | qPCR primers | AGATGTGGATCAGCAAGCAG |
| Sequence-based reagent | b-actin-R | Sigma | qPCR primers | GCGCAAGTTAGGTTTTGTCA |
| Sequence-based reagent | Col1a1-F | Sigma | qPCR primers | GCATGGCCAAGAAGACATCC |
| Sequence-based reagent | Col1a1-R | Sigma | qPCR primers | CCTCGGGTTTCCACGTCTC |
| Sequence-based reagent | Adgrg6-F | Sigma | qPCR primers | CCAAAGTTGGCAATGAAGGT |
| Sequence-based reagent | Adgrg6-R | Sigma | qPCR primers | GCTGGATCAGGTAGGAACCA |
| Sequence-based reagent | Col10a1-F | Sigma | qPCR primers | CTTTGTGTGCCTTTCAATCG |
| Sequence-based reagent | Col10a1-R | Sigma | qPCR primers | GTGAGGTACAGCCTACCAGTTTT |
| Sequence-based reagent | Col2a1-F | Sigma | qPCR primers | ACTGGTAAGTGGGGCAAGAC |
| Sequence-based reagent | Col2a1-R | Sigma | qPCR primers | CCACACCAAATTCCTGTTCA |
| Sequence-based reagent | Acan-F | Sigma | qPCR primers | CGTGTTTCCAAGGAAAAGGA |
| Sequence-based reagent | Acan-R | Sigma | qPCR primers | TGTGCTGATCAAAGTCCAG |
| Sequence-based reagent | Sox9-F | Sigma | qPCR primers | AGGAAGCTGGCAGACCAGTA |
| Sequence-based reagent | Sox9-R | Sigma | qPCR primers | CGTTCTTCACCGACTTCCTC |
| Chemical compound, drug | Forskolin | Sigma | Sigma: F6886 | |
| Software, algorithm | GraphPad Prism software | GraphPad Prism (https://graphpad.com) |