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. 2021 Aug 3;799:149405. doi: 10.1016/j.scitotenv.2021.149405

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© 2021 Published by Elsevier B.V.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 2 — Concentrating SARS-CoV-2 from wastewater samples via centrifugation or vacuum filtration without centrifugation. WWTP: South Austin Regional (SAR); Sampling location: Primary Clarifier Effluent (PCE); Sample details: six composite samples were retrieved on six different dates between November and December 2020; Pasteurization: yes; Concentration Method: centrifugation vs. vacuum filtration without centrifugation; RNA Extraction Method: MagMax with zirconia lysis beads for samples concentrated via centrifugation or garnet lysis beads for samples concentrated via vacuum filtration without centrifugation. Samples concentrated via centrifugation were processed using the final selected workflow. Each column represents the average of triplicate aliquots processed for a sample, where triplicate RT-qPCR reactions were run for each aliquot; error bars represent the standard deviation among the aliquots; the coefficient of variation (CV) normalizes the standard deviation to the average SARS-CoV-2 concentration.