Fig. 3.

Concentrating SARS-CoV-2 from wastewater samples taken following primary treatment (PCE) or preliminary treatment (INF). WWTP: South Austin Regional (SAR); Sampling location: Primary Clarifier Effluent (PCE) and Influent (INF); Sample details: two pairs of PCE and INF composite samples were retrieved on two different dates between November and December 2020; Pasteurization: yes; Concentration Method: centrifugation; RNA Extraction Method: MagMax with zirconia lysis beads. PCE samples were processed using the final selected workflow. Each column represents the average of triplicate aliquots processed for a sample, where triplicate RT-qPCR reactions were run for each aliquot; error bars represent the standard deviation among the aliquots; the coefficient of variation (CV) normalizes the standard deviation to the average SARS-CoV-2 concentration. A CV value noted as “N/A” indicates that two of the triplicate aliquots processed yielded triplicate non-detect RT-qPCR results.