Figure 2. Inoculation of perennial ryegrass seedlings and plant cultivation.

A. Set up a dissecting microscope under a laminar flow cabinet. B. Inoculate a small piece of E. festucae mycelium into a small incision made at the SAM, visible as a small knot. C and D. Two weeks after inoculation (one week in the dark, one week in light) plant seedlings in sterile jars or in root trainers in potting mix. E. Check for infection when several tillers have developed.