| Antigen | Fluorophore | Volume |
| CD45 | BV510 | 2.5 μl |
| CD14 | AF700 | 0.5 μl |
| CD16 | PE-CF594 | 1 μl |
| CD19 | PE | 2 μl |
| CD3 | BV650 | 2.5 μl |
| CD8 | FITC | 1.25 μl |
| CD4 | BV605 | 2 μl |
| CD56 | PerCPCy5.5 | 5 μl |
| HLA-DR | BV785 | 2.5 μl |
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