Figure 4. 2D-BN-PAGE separation of thylakoid protein complexes.

Protein complexes B1-B9 (green bands on the upper horizontal gel slice) were first separated according to their mass and shape on 1D-BN-PAGE. After separation the gel slice was subjected to 2D-BN-PAGE during which the complexes (B1-B9) are fractionated into subcomplexes (narrow green bands on the 2D-BN-gel). The complexes on the diagonal (dashed line) represent complexes that have preserved their mass, whereas the complexes below the diagonal are subcomplexes (of B1-B9) that have been disconnected during second solubilization and 2D-BN-PAGE.