Figure 6. The representative results from the analysis of the subcomplex and subunit composition of thylakoid protein complexes.

A. Protein complexes (B1-B9) of digitonin solubilized thylakoids after 1D-BN-PAGE separation. B. 2D-BN-PAGE separation of the subcomplexes obtained by re-solubilizing the 1D-strip with β-DM. Ctrl represents thylakoids directly solubilized with 1% DM. C. The Lhcb-protein localization in protein subcomplexes was analyzed by electroblotting the 2D-BN-gels and immunodecorating the blots with Lhcb1, Lhcb2, Lhcb3 protein specific antibodies and with Phospho-Lhcb1-2 protein specific antibodies. D. The protein composition of the subcomplexes (derived from complexes B1-B9) was analyzed by cutting the lanes from 2D-BN-gel and subjecting the gel strips to 3D-BN-PAGE. The proteins are visualized with Sypro Ruby stain. This figure has been originally published in Rantala et al. (2017) .