Light | Heavy | Stack | |
48% AA, 1.5% bis-AA | 148 µl | 530 µl | - |
20% AA, 5% bis-AA | - | - | 180 µl |
3x gel buffer | 700 µl | 700 µl | 500 µl |
75% glycerol | 140 µl | 560 µl | - |
H2O | 1,092 µl | 290 µl | 800 µl |
5% APS | 15 µl | 11 µl | 30 µl |
TEMED | 3 µl | 2 µl | 3 µl |
Pipet to the chamber | 1,830 µl | 1,880 µl |