Light	Heavy	Stack
48% AA, 1.5% bis-AA	148 µl	530 µl	-
20% AA, 5% bis-AA	-	-	180 µl
3x gel buffer	700 µl	700 µl	500 µl
75% glycerol	140 µl	560 µl	-
H2O	1,092 µl	290 µl	800 µl
5% APS	15 µl	11 µl	30 µl
TEMED	3 µl	2 µl	3 µl
Pipet to the chamber	1,830 µl	1,880 µl