| Titrant (CPP, 500 μM) | Titrated molecule (14-3-3c, 50 μM) |
|---|---|
| --- | 1,000 μl 14-3-3c (stock 100 μM) |
|
16.7 μl (stock 15 mM in MilliQ water)
1/30 of the total volume
|
66.7 μl MilliQ water
1/30 of the total volume
|
| 483.3 μl buffer (see Recipes) | 933.3 μl buffer (see Recipes) |
| Total volume: 500 μl | Total volume: 2,000 μl |
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