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. 2021 Jul 24;2021:9813773. doi: 10.1155/2021/9813773

Figure 2.

Figure 2

The exosomal loading of miR-665 relies on the facilitation of SCIRT and hnRNPA1. (a) Predicted binding sites between miR-665 and lncRNA SCIRT. (b) Western blotting analysis of CD63, TSG101, and calnexin in exosomes extracted from the cell culture supernatants and lung cancer cells. RT-qPCR analysis of the expression of (c) miR-665 and (d) SCIRT in cancer-cell-derived exosomes or lung cancer cells. (e) Knockdown of SCIRT with specific siRNAs in lung cancer cells. (f) RT-qPCR analysis of miR-665 expression after SCIRT silencing. (g) RT-qPCR analysis of the expression of miR-665 and SCIRT in H446 cells after hnRNPA1 silencing. The analyses were repeated three times, and the results were expressed as mean ± SD. ∗P < 0.05 and ∗∗P < 0.01.