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. 2021 Jan 25;16(9):1677–1685. doi: 10.4103/1673-5374.306093

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Characterization of NSCs from newborn rat hippocampus.

(A, B) Phase contrast microscopy images of a neurosphere in NSC culture for 8 days (A) and a nestin-positive (green, Cy3) neurosphere (B). Nuclei were labeled by Hoechst 33258 (blue). (C, D) NSCs differentiated into MAP2-positive neurons (C; red, Cy3), GFAP-positive cells (C, D; green, FITC), and Galc-positive oligodendrocytes (D; red, Cy3) in differentiation medium for 8 days. Nuclei were labeled by Hoechst 33258 (blue). Scale bars: 50 μm in A and B, 100 μm in C and D. Galc: Galactose cerebroside; GFAP: glial fibrillary acidic protein; MAP2: microtubule associated protein 2; NSC: neural stem cell.