Figure 3.

Identification of primary cultured mixed glia, astrocytes and microglia.

(A) Phase-contrast micrographs showing mixed glial cells (left), microglia (middle), and astrocytes (right). Solid arrows indicate astrocytes; dotted arrows indicate microglia. (B) Microglia in microglia culture stained with GSI-B4-FITC (B, green). (C) Astrocytes in astrocyte culture stained with GFAP antibody (C, red, Cy3). Nuclei were labeled by Hoechst 33258 (blue). Scale bars: 50 μm in A, 100 μm in B and C. (D) Quantitation of the purity of microglia and astrocytes. Data are expressed as the mean ± SEM from three independent experiments. GFAP: Glial fibrillary acidic protein.