Figure 4.

Protein expression changes in HMGB1, TLR4, COXs, and PGESs in the injured spinal cord of rats following spinal cord injury.

(A) Western blot analysis of HMGB1, TLR4, COX1, COX2, mPGES-1, mPGES-2, and cPGES following spinal cord contusion at 0, 1, 4, and 7 days. β-Actin was used as an internal control. (B–H) Quantification data of relative expression of HMGB1 (B), TLR4 (C), COX1 (D), COX2 (E), mPGES-1 (F), mPGES-2 (G), and cPGES (H). Relative expression is expressed as the optical density ratio to β-actin. Experiments were performed in triplicate. Data are expressed as the mean ± SEM (n = 6). *P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc test). COX: Cyclooxygenase; cPGES: cytosolic prostaglandin E synthase; HMGB1: high mobility group box 1; mPGES: microsomal prostaglandin E synthase; TLR4: Toll-like receptor 4.