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. 2021 Jan 25;16(9):1848–1855. doi: 10.4103/1673-5374.303039

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Copyright: © 2021 Neural Regeneration Research

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Co-localization of TLR4 and COX2 with astrocytes in the injured spinal cord of rats following SCI.

(A) Illustration of section sites showing immunofluorescence. The rectangle indicates the observed field. (B) Immunofluorescence analysis of TLR4 (red, stained by Cy3) co-localization with GFAP-positive cells (green, stained by fluorescein isothiocyanate) in the injured spinal segments following SCI at 0 and 4 days. (C) Immunofluorescence analysis of COX2 (red, stained by Cy3) co-localization with GFAP-positive cells (green, stained by fluorescein isothiocyanate) in injured spinal segments following SCI at 0 and 4 days. The rectangle indicates the magnified region. Scale bars: 50 μm. COX2: Cyclooxygenase 2; GFAP: glial fibrillary acidic protein; SCI: spinal cord injury; TLR4: Toll-like receptor 4.