Figure 7.

Effects of VX-765 on Th1, Th2, and Th17 in the injured spinal cord at 7 days post-injury: immunofluorescence detection.

(A–I) CD4 (green, stained by FITC) and T-bet (red, stained by rhodamine) (A, D, and G) for Th1 cells, or GATA3 (red, stained by rhodamine) (B, E, and H) for Th2 cells, or RORγ-t (red, stained by rhodamine) (C, F, and I) for Th17 cells, in the different groups. Nuclei are blue, stained by Hoechst 33342. Scale bars: 400 µm, 20 µm (enlarged parts). (J–L) CD4⁺ T-bet⁺ Th1 (J), CD4⁺GATA3⁺ Th2 (K), and CD4⁺RORγ-t⁺ Th17 (L) cell counts in the sham, DMSO, and VX-765 groups. The original data for Figure J–L are shown in Additional file 7. Data are represented as the mean ± SD (n = 6). *P < 0.05, **P < 0.01 (non-parametric Kruskal–Wallis analysis of variance). DMSO: Dimethyl sulfoxide; FITC: fluoresceine isothiocyanate; GATA3: GATA-binding protein 3; SCI: spinal cord injury; RORγ-t: retinoid-related orphan nuclear receptor γ-t; T-bet: Th1-specific T box transcription factor; Th: T helper; VX-765: caspase-1 selective inhibitor.