Skip to main content
. 2021 Jan 25;16(9):1836–1847. doi: 10.4103/1673-5374.306096

Additional Table 2.

Information of antibodies used in flow cytometry

Antigen Host species and clone Cat#or Lot# RRID Conjugation Source Used concentration
CCR7 Rat monoclonal 47-1971-82 AB_2573974 AF780 Invitrogen 0.25μg/test
IgG2bκ isotype control Rat 47-4321-82 AB_1271997 0.25μg/test
CD11b Rat monoclonal 12-0112-81 AB_465546 PE 0.125 μg/test
CD3 Rat monoclonal 12-0032-82 AB_2811741 0.25μg/test
IgG2bκ isotype control Rat 12-4031-82 AB_470042 0.25μg/test
CD127 Rat monoclonal 48-1273-82 AB_2574039 eFlour 450 0.5 μg/test
IgG2b κ isotype control Rat 48-4031-82 AB_1272017 0.5 μg/test
CD183 Armenian hamster monoclonal 62-1831-82 AB_2762747 Super Bright 436 0.5 μg/test
IgG isotype control Armenian hamster 62-4888-82 AB_2717007 0.5 μg/test
CD196 Rat monoclonal 50-7196-82 AB_11219682 eFlour 660 0.5 μg/test
IgG2a κ isotype control Rat 50-4321-82 AB_10598503 0.5 μg/test
CD25 Rat monoclonal 47-0251-82 AB_1272179 AF780 0.5 μg/test
IgG1 κ Isotype Control Rat 47-4301-80 AB_1271986 0.5 μg/test
CD28 Syrian hamster Monoclonal 45-0281-80 AB_925744 PerCP-Cyanine5.5 0.5 μg/test
IgG isotype control Armenian hamster 45-4888-80 AB_906260 0.5 μg/test
CD3 Armenian hamster monoclonal 47-0031-82 AB_11149861 AF780 0.5 μg/test
IgG Isotype Control Armenian hamster 47-4888-80 AB_1271978 0.5 μg/test
CD4 Rat monoclonal 11-0041-82 AB_464892 FITC 0.25μg/test
CD68 Rat monoclonal MA5-16676 AB_2538170 0.25μg/test
IgG2bκ isotype control Rat 11-4031-82 AB_470004 0.25μg/test
CD45 Rat monoclonal 17-0451-82 AB_469392 APC 0.125 μg/test
IgG2b κ Isotype Control Rat 17-4031-82 AB_470176 0.125 μg/test

AF780: APC-eFluor 780; APC: allophycocyanin; CCR7: chemokine (C-C motif) receptor 7; FITC: fluoresceine isothiocyanate; PE: phycoerythrin.