Additional Table 2.
Antigen | Host species and clone | Cat#or Lot# | RRID | Conjugation | Source | Used concentration |
---|---|---|---|---|---|---|
CCR7 | Rat monoclonal | 47-1971-82 | AB_2573974 | AF780 | Invitrogen | 0.25μg/test |
IgG2bκ isotype control | Rat | 47-4321-82 | AB_1271997 | 0.25μg/test | ||
CD11b | Rat monoclonal | 12-0112-81 | AB_465546 | PE | 0.125 μg/test | |
CD3 | Rat monoclonal | 12-0032-82 | AB_2811741 | 0.25μg/test | ||
IgG2bκ isotype control | Rat | 12-4031-82 | AB_470042 | 0.25μg/test | ||
CD127 | Rat monoclonal | 48-1273-82 | AB_2574039 | eFlour 450 | 0.5 μg/test | |
IgG2b κ isotype control | Rat | 48-4031-82 | AB_1272017 | 0.5 μg/test | ||
CD183 | Armenian hamster monoclonal | 62-1831-82 | AB_2762747 | Super Bright 436 | 0.5 μg/test | |
IgG isotype control | Armenian hamster | 62-4888-82 | AB_2717007 | 0.5 μg/test | ||
CD196 | Rat monoclonal | 50-7196-82 | AB_11219682 | eFlour 660 | 0.5 μg/test | |
IgG2a κ isotype control | Rat | 50-4321-82 | AB_10598503 | 0.5 μg/test | ||
CD25 | Rat monoclonal | 47-0251-82 | AB_1272179 | AF780 | 0.5 μg/test | |
IgG1 κ Isotype Control | Rat | 47-4301-80 | AB_1271986 | 0.5 μg/test | ||
CD28 | Syrian hamster Monoclonal | 45-0281-80 | AB_925744 | PerCP-Cyanine5.5 | 0.5 μg/test | |
IgG isotype control | Armenian hamster | 45-4888-80 | AB_906260 | 0.5 μg/test | ||
CD3 | Armenian hamster monoclonal | 47-0031-82 | AB_11149861 | AF780 | 0.5 μg/test | |
IgG Isotype Control | Armenian hamster | 47-4888-80 | AB_1271978 | 0.5 μg/test | ||
CD4 | Rat monoclonal | 11-0041-82 | AB_464892 | FITC | 0.25μg/test | |
CD68 | Rat monoclonal | MA5-16676 | AB_2538170 | 0.25μg/test | ||
IgG2bκ isotype control | Rat | 11-4031-82 | AB_470004 | 0.25μg/test | ||
CD45 | Rat monoclonal | 17-0451-82 | AB_469392 | APC | 0.125 μg/test | |
IgG2b κ Isotype Control | Rat | 17-4031-82 | AB_470176 | 0.125 μg/test |
AF780: APC-eFluor 780; APC: allophycocyanin; CCR7: chemokine (C-C motif) receptor 7; FITC: fluoresceine isothiocyanate; PE: phycoerythrin.