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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Transl Res. 2021 Apr 20;235:115–128. doi: 10.1016/j.trsl.2021.04.001

Figure 3: ASK1 inhibition does not improve monolayer permeability of endothelial cells treated with LPS.

Figure 3:

Transendothelial electrical resistance (TEER) of HMVECs pretreated with either GS444217 or MSC2032964A (A) at 5 or 25 μM prior to addition of LPS (100 ng/ml) or vehicle control. All data is normalized to baseline resistance per condition prior to addition of LPS. Arrows indicate time of inhibitor (or vehicle) and LPS (or control) given. B) Area under the curve relative to change from baseline resistance over time calculated for all conditions following LPS addition out to 18h of data (n = 5). C) Representative immunofluorescence images used for quantification of intercellular area (D) and VE-cadherin (E) staining following LPS in the presence or absence of either GS444217 or MSC2032964A (green = intercellular space, red = VE-cadherin, blue = nuclei). * = p < 0.05 between designated groups. NS = non-significant.