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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Transl Res. 2021 Apr 20;235:115–128. doi: 10.1016/j.trsl.2021.04.001

Figure 5: ASK1 directly interacts with eNOS without affecting LPS-induced eNOS-p38 interaction.

Figure 5:

A) PLA for eNOS/ASK1 interaction with indicated siRNA in representative confocal images (40x, line bar represents a distance of 50 μm, green = PLA signal, blue = nuclei). Calculation of relative eNOS/ASK1 PLA signal (as determined by total fluorescence intensity) either in the presence or absence of 100 ng/ml of LPS (B) or 1 μM of GS444217 (C) for 1 hour (n = 5 individual replicate per group). D) eNOS/p-p38 PLA signal at 0, 30, and 60 minutes calculated relative to vehicle control at the time of LPS addition (n = 6 per group). * = p < 0.05 between respective groups. † = p < 0.05 compared to vehicle control at time 0. NS = non-significant.