Figure 2. Glucocorticoids are required to suppress spontaneous SPEM in females but are dispensable in males.

(A) Immunostaining for the SPEM marker CD44 variant 9 (green), mucous neck cells are stained with GSII lectin (white). Nuclei are stained with DAPI. Scale bars are 100 µm. (B) Quantitative RT-PCR of the indicated SPEM marker genes using RNA isolated from the gastric corpus (n≥4 mice/group). Data are mean ± SD; P-values were determined by one-way ANOVA with post hoc Tukey’s t-test. * P≤0.01 **P≤0.001 ***P≤0.0001