Skip to main content
. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Gastroenterology. 2021 May 7;161(2):637–652.e4. doi: 10.1053/j.gastro.2021.04.075

Figure 3. Androgens suppress gastric inflammation and SPEM following adrenalectomy.

Figure 3.

(A) Experimental model: Female mice were adrenalectomized and simultaneously implanted with a sustained-release 5α dihydrotestosterone (DHT) pellet. Stomachs were collected 2 months after surgery. (B) Immunostaining of stomach sections probed for CD45 (pan-leukocytes, green), for ATP4B (parietal cells, red), GSII lectin (mucous neck cells, white), and MIST1(chief cells, green), or for CD44v9 (SPEM, green) and GSII lectin (white). Nuclei are stained with DAPI. Scale bars are 100 µm. (C) Quantitation of the number of parietal cells and chief cells observed per 20x field (n≥5 mice/group). Data are mean ± SD; P-values were determined by one-way ANOVA with post hoc Tukey’s t-test. ***P≤0.0001