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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Gastroenterology. 2021 May 7;161(2):637–652.e4. doi: 10.1053/j.gastro.2021.04.075

Figure 4. Androgen treatment reverses chronic SPEM in female mice.

Figure 4.

(A) Experimental design. Female mice were aged 1 month after sham surgery or adrenalectomy. They were then implanted with a sustained release dihydrotestosterone pellet or placebo controls and then were aged for an additional month. (B) Immunostaining of stomach sections probed for (B) ATP4B (parietal cells, red), GSII lectin (mucous neck cells, white), and MIST1(chief cells, green) or for (C) CD44v9 (SPEM, green). Nuclei are stained with DAPI. Scale bars are 100 µm (D) Quantitation of the number of parietal cells and chief cells observed per 20x field (n≥5 mice/group). Data are mean ± SD; P-values were determined by one-way ANOVA with post hoc Tukey’s t-test. ***P≤0.0001