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. 2021 Feb 9;113(8):1094–1104. doi: 10.1093/jnci/djab018

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Figure 3. — RNA-Seq analysis of glioma cells treated with quetiapine and radiation. (A) Volcano diagrams of differentially expressed genes (DEG) in HK-374 cells, 48 hours after treatment with radiation (IR, 4 Gy, RT), quetiapine (QTP), or quetiapine and radiation (Comb) compared with untreated control (Cnt) cells. (B) Venn diagrams of differentially expressed genes in HK-374 cells, 48 hours after treatment with radiation, quetiapine, or quetiapine and radiation. (C) Heatmap of differential expression genes in HK-374 cells, 48 hours after treatment with radiation, quetiapine, or quetiapine and radiation. (D) Top 10 up- and downregulated genes and their validation by qRT-PCR (E). Top 20 gene ontology gene set overlapping with genes differentially up- or downregulated in HK-374 cells, 48 hours after treatment with radiation and quetiapine (F). Genes involved in the biosynthesis of cholesterol are upregulated in HK-374 (G), HK-217, and HK-382 (H) cells, 48 hours after treatment with radiation (4 Gy) and quetiapine or radiation. Genes of the first-level regulator network of SREBF2, the master regulator of cholesterol biosynthesis, are upregulated in HK-374 (I), HK-217, and HK-382 (J) cells, 48 hours after treatment with radiation (4 Gy) and quetiapine or radiation.

Figure 3. — RNA-Seq analysis of glioma cells treated with quetiapine and radiation. (A) Volcano diagrams of differentially expressed genes (DEG) in HK-374 cells, 48 hours after treatment with radiation (IR, 4 Gy, RT), quetiapine (QTP), or quetiapine and radiation (Comb) compared with untreated control (Cnt) cells. (B) Venn diagrams of differentially expressed genes in HK-374 cells, 48 hours after treatment with radiation, quetiapine, or quetiapine and radiation. (C) Heatmap of differential expression genes in HK-374 cells, 48 hours after treatment with radiation, quetiapine, or quetiapine and radiation. (D) Top 10 up- and downregulated genes and their validation by qRT-PCR (E). Top 20 gene ontology gene set overlapping with genes differentially up- or downregulated in HK-374 cells, 48 hours after treatment with radiation and quetiapine (F). Genes involved in the biosynthesis of cholesterol are upregulated in HK-374 (G), HK-217, and HK-382 (H) cells, 48 hours after treatment with radiation (4 Gy) and quetiapine or radiation. Genes of the first-level regulator network of SREBF2, the master regulator of cholesterol biosynthesis, are upregulated in HK-374 (I), HK-217, and HK-382 (J) cells, 48 hours after treatment with radiation (4 Gy) and quetiapine or radiation.