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. 2021 Aug 2;12(8):761. doi: 10.1038/s41419-021-04055-2

Fig. 4. mTORC1 enhances FGFBP1 expression through activation of STAT3.

Fig. 4

A, B NCI-H292 (A) and BEAS-2B (B) cells were pretreated with PLA (20 μg/mL, 12 h) before the addition of S3I-201 (100 nM) for 12 h. C, D NCI-H292 (C) and BEAS-2B (D) cells, stably expressed lentivirus shSTAT3-1, shSTAT3-2, or shSc, were treated with or without PLA (20 μg/mL, 24 h). E, F NCI-H292 (E) and BEAS-2B (F) cells were transfected with pBabe-STAT3C, a constitutively active STAT3 mutant, or the control vector pBabe. G, H siRNAs against STAT3 (siSTAT3) or the control (siNC) were transfected into Tsc1−/− MEFs (G) and Tsc2−/− MEFs (H). A–H Cell lysates of the indicated cells were harvested for immunoblot analysis with the indicated antibodies. All experiments were performed in triplicate.