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. 2021 Aug 2;11:15655. doi: 10.1038/s41598-021-95165-6

Figure 1.

Figure 1

Expression of transcription variants (TVs) in MCF7 and HL60 cells. (a) The CXXC5 gene contains 11 exons and 10 introns. (b) A screenshot of transcript annotations by Genome Data Viewer of NCBI indicates the presence of 14 transcript variants encoded by the CXXC5 gene. (c) The presence of CXXC5-transcript variants in MCF7 and HL60 cells was assessed with multiple rounds of PCRs with progressively nested primers specific to a variant together with a primer specific to Exon10, which is common to all CXXC5-transcript variants, followed by cloning and sequencing of the PCR amplicons. (d) Screenshots of the expression levels of CXXC5-transcript variants in healthy breast tissue and blood annotated by GTEx Portal. (e) qPCR of cDNA libraries was used to assess the expression levels of transcript variants in MCF7 and HL60 cells using primer sets also utilized in evaluating the presence of transcript variants. Asterisk (*) indicates the highest expression among transcript variants. (f) Northern blot analyses of RNA samples from MCF7 or HL60 cells. A biotinylated probe complementary to Exon10/11 present in all transcript variants and a biotinylated probe targeting Exon3 were used for the detection of the CXXC5 transcripts. A GAPDH targeting probe was also used for the detection of the GAPDH transcripts as control. The molecular ladder (nt) is indicated.