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. 2021 Aug 2;12:4664. doi: 10.1038/s41467-021-25015-6

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — HEK293T cells (a) and A549 cells (b) were co-transfected with GFP/HA-NLRP3, GFP-N/HA-NLRP3, GFP/Flag-ASC, GFP-N/Flag-ASC, GFP/HA-NLRP3/Flag-ASC, GFP-N/HA-NLRP3/Flag-ASC, and GFP-3a/HA-NLRP3/Flag-ASC for 24 h. The sub-cellular locations of HA-tagged NLRP3 (red), Flag-tagged ASC (yellow), GFP-tagged N or GFP-tagged 3a (green), and nucleus marker DAPI (blue) were visualized with confocal microscopy. GFP-NC means pEGFP-C1 empty plasmid (a, b). Data are representative of three independent experiments. Source data are provided as a Source Data file.