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. 2021 Jul 31;7:199. doi: 10.1038/s41420-021-00583-0

Fig. 2. RASSF9 increases the expression of cyclins and oncogenes.

Fig. 2

a Elevated expression of RASSF9 increases the mRNA levels of cyclins in A549, H1299, and H1650 cells. b Effects of RASSF9 on the expression of c-Myc, Pax, and Fos. Cells were transfected with the plasmid expressing Rassf9. Control cells were transfected with the empty vector pcDNA3.1 (EV). Twenty-four hours post-transfection, total RNA was extracted for gene expression analysis by qRT-PCR, and GAPDH was used as a house-keeping gene. Data are presented as means ± SD (error bars). *P < 0.05, **P < 0.01, and ***P < 0.001. Student’s t-test.