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. 2021 Aug 2;11:15618. doi: 10.1038/s41598-021-93381-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Estimation of the probability of RNA-Seq data from hybrid species. The bars show the distribution of ratio of nucleotide sites expressing two different alleles (mixed site). Mixed RNA-Seq samples are generated by pair-wise mixing RNA-Seq data of 16 Flaveria species, which mimics the case of hybridization. The ratio of mixed site in the mixed RNA-Seq samples is showed in grey bars (positive control). The ratio of mixed site of 16 Flaveria species is showed in red bars (real causes). The ratio of hybrid sample F. pringlei*, was represented in blue vertical line.