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. 2021 Aug 2;11:15618. doi: 10.1038/s41598-021-93381-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Modifications in the predicted amino acid sequences of proteins involved in cyclic electron transport and transcript abundances of the cognate transcripts mapped to the Flaveria phylogeny. Changes in predicted amino acid sequence in proteins involved in cyclic electron transport chain and abundances (FPKM) of their cognate transcripts in C₄ and C₃ Flaveria species are shown. Only the amino acid residues predicted to be different between C₃ and C₄ species are superimposed recent published phylogeny of Flaveria. The marked colors of amino acid residues have no meaning and are only for visualization purposes. Numbers below the amino acids indicate the location site in the multiple sequence alignments. FPKM values with standard errors are represented to the right of the amino acid changes as red bars. (A) Protein gradient regulation 5 like protein (PGR5-like); (B) NADH dehydrogenase-like (Ndh) L2 subunit (Ndh L2); (C) NdhV; (D) Ndh16; (E) NdhU; (F) NdhM; (G) Ndh48; (H) NdhB4; (I) chlororespiration reduction 1 (CRR1). The sequence alignments are available in Additional file 2.