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. 2021 Jun 25;38(2):277–283. doi: 10.5511/plantbiotechnology.21.0422a

Figure 3. Comparison of the germinability (A) and the electrophoresis patterns of total RNA extracted from the embryos (B) of fresh and aged seeds in rice cultivars Nipponbare, Sasanishiki and Koshihikari. The aged seeds of Nipponbare, Sasanishiki and Koshihikari were harvested in 2010, 2009 and 2007, respectively, after cultivation under natural conditions in Toyama Prefecture, Japan. Fresh seeds from three cultivars were harvested in 2017. (A) Fresh (white bars) and aged (black bars) seeds were incubated in petri dishes with distilled water at 28°C for ten days. Values of germination rate are means of three replicates±SE Student’s t-test was used to compare the mean (n=3) germination rate (%) of seeds for each cultivar (**: p<0.01). (B) The extracted total RNAs (each 200 ng) from the embryos of fresh and aged seeds were analyzed by electrophoresis using an Agilent 2100 Bioanalyzer system.

Figure 3. Comparison of the germinability (A) and the electrophoresis patterns of total RNA extracted from the embryos (B) of fresh and aged seeds in rice cultivars Nipponbare, Sasanishiki and Koshihikari. The aged seeds of Nipponbare, Sasanishiki and Koshihikari were harvested in 2010, 2009 and 2007, respectively, after cultivation under natural conditions in Toyama Prefecture, Japan. Fresh seeds from three cultivars were harvested in 2017. (A) Fresh (white bars) and aged (black bars) seeds were incubated in petri dishes with distilled water at 28°C for ten days. Values of germination rate are means of three replicates±SE Student’s t-test was used to compare the mean (n=3) germination rate (%) of seeds for each cultivar (**: p<0.01). (B) The extracted total RNAs (each 200 ng) from the embryos of fresh and aged seeds were analyzed by electrophoresis using an Agilent 2100 Bioanalyzer system.