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. 2021 Jun 25;38(2):187–196. doi: 10.5511/plantbiotechnology.21.0121a

Figure 4. Detection of donor-derived sRNAs in the recipient plant by stem-loop PCR. (A) Cuscuta campestris (donor)-derived sRNAs in Arabidopsis thaliana (recipient). (B) A. thaliana (donor)-derived sRNAs in C. campestris (recipient). (C) Detection of CcsRNA0641, and absence of CcsRNA4295 in leaf, stem, and root of A. thaliana (recipient). CCNS, nonparasitic stem of C. campestris; CCPS, parasitic stem of C. campestris; CCNSap, nonparasitic stem of C. campestris containing apical region; CCPSap, parasitic stem of C. campestris containing apical region; IPS, parasitic interface; ATNS, nonparasitized stem of A. thaliana; ATPS, parasitized stem of A. thaliana; ATNSap, apical region of ATNS; ATPSap, apical region of ATPS; NC, no template control. Numbers on top of the lanes indicate replicates. Open arrowheads indicate that no amplification products were detected. ND, not detected.

Figure 4. Detection of donor-derived sRNAs in the recipient plant by stem-loop PCR. (A) Cuscuta campestris (donor)-derived sRNAs in Arabidopsis thaliana (recipient). (B) A. thaliana (donor)-derived sRNAs in C. campestris (recipient). (C) Detection of CcsRNA0641, and absence of CcsRNA4295 in leaf, stem, and root of A. thaliana (recipient). CCNS, nonparasitic stem of C. campestris; CCPS, parasitic stem of C. campestris; CCNSap, nonparasitic stem of C. campestris containing apical region; CCPSap, parasitic stem of C. campestris containing apical region; IPS, parasitic interface; ATNS, nonparasitized stem of A. thaliana; ATPS, parasitized stem of A. thaliana; ATNSap, apical region of ATNS; ATPSap, apical region of ATPS; NC, no template control. Numbers on top of the lanes indicate replicates. Open arrowheads indicate that no amplification products were detected. ND, not detected.