Table 1. Mobile sRNA candidates and summary of the trans-species mobility.
sRNA ID | Sequence, Donor genomic loci, Description | Length (nt) | Length of SL- PCRa (nt) | Av RPM in donor PSb | Mobilityc |
---|---|---|---|---|---|
CcsRNA0613 | ACUGCGAUUCCUACUUCUGCCA | 22 | 62 | 192.3 | N |
Ccam0.32_scaffold87b:766744-766765 | |||||
Cc015853 SNF2 domain protein | |||||
CcsRNA0641 | ACUUGCGUUCAAAGUUUCGAU | 21 | 61 | 358.3 | M |
Ccam0.32_scaffold327b:26842-26992 | |||||
Non-coding region | |||||
CcsRNA0897 | AGGGGCGAAAGACUAAUCGAACC | 23 | 63 | 420.7 | N |
Ccam0.32_scaffold428:135804-135828 | |||||
Non-coding region | |||||
CcsRNA1003 | AGUUUUUCCACUCGCCAACUCGC | 23 | 63 | 245.7 | N |
Ccam0.32_scaffold158:308476-308499 | |||||
Reverse transcriptase | |||||
CcsRNA3151 | GAUGCUCUUCCAACCGCUGGAC | 22 | 62 | 93.0 | N |
Ccam0.32_scaffold490:38023-38045 | |||||
RNase H family protein | |||||
CcsRNA4295 | GUUUGAAUUGUAGUCUGGAGA | 21 | 61 | 458.3 | M |
Ccam0.32_scaffold3003:6576-6597 | |||||
Non-coding region | |||||
CcsRNA5627 | UUUGAAUUGUAGUCUGGAGA | 20 | 60 | 405.3 | N |
Ccam0.32_scaffold757:3409-3433 | |||||
Non-coding region | |||||
AtsRNA1127 | ACCCGUUAAUGACUGUUAAUCUGU | 24 | 64 | 60.0 | N |
Chr5:8313181-8313483 | |||||
AT5G24352 protein kinase | |||||
AtsRNA2146 | AUAAAACAUGAUCAAAGGGUG | 21 | 61 | 43.0 | N |
Chr5:9741714-9741938 | |||||
Non-coding region | |||||
AtsRNA3031 | CAUCCGUUGACCAUGUUUUAU | 21 | 61 | 48.5 | M |
Chr3:8842983-8843011 | |||||
Non-coding region | |||||
AtsRNA4110 | GAGUUUUUCAGUAUCUGUUCUU | 22 | 62 | 21.0 | N |
Chr1:11793991-11794171 | |||||
AT1G32610 glycoprotein family protein | |||||
AtsRNA4348 | GCCCAUUUUAAACCUGUGUU | 20 | 60 | 8.0 | M |
Chr1:13152671-13152836 | |||||
Non-coding region | |||||
AtsRNA4501 | GCUUUCUCCAAUCUGCUAGAA | 21 | 61 | 16.5 | N |
Chr2:16467151-16467318 | |||||
AT2G39440 ribonuclease H2 subunit C |
aLength of stem-loop RT-PCR product. bAverage reads per million (RPM) in the parasitic- or parasitized stems of the donor plants. cExperimentally examined mobility to the recipient plant in this study; M, mobile; N, not mobile.