Table 1. Sequences of the mono and biallelic indels in gfp transgene induced by CRISPR/Cas9 NHEJ system, gfp mRNA expression level, GFP protein accumulation, and segregation behavior of the CRISPR/Cas9 transgene in gene edited plants from events EC1 to EC10 compared to parental unedited gfp transgenic plants (ECWT1 and ECWT2).

Events	Decoding of mono and biallelic mutations	Decoding results	gfp mRNA accumulation	GFP protein accumulation	Segregation ratio in T1
ECWT1	GGATGGTGATGTGAACGGGCAC	Non-edited control lineage 1	Positive	Positive	—
ECWT2	GGATGGTGATGTGAACGGGCAC	Non-edited control lineage 2	Positive	Positive	—
EC1	ECWT line GGAACTGGATGGTGATGTGAACGGGCAC- EC1 line GGAACTGGATGGTGAT-KKRAMSGGCMCM Allele 1 GGAACTGGATGGTGAT TGAACGGGCACA ****************-************ Allele 2 GGAACTGGATGGTGATGTGAACGGGCAC ****************************	Allele 1: deletion of G nucleotide; Allele 2: no indels; Monoallelic mutant.	Positive	Positive	3 : 1
EC2	ECWT line GGAACTGGATGGTGATG-TGAACGGGCAC EC2 line GGAACTGGATGGTGATGWWSMRSSRSMA- Allele 1 GGAACTGGATGGTGATGATGAACGGGCA- *****************-**********- Allele 2 GGAACTGGATGGTGATGT--ACGGGCACAA ******************--**********	Allele 1: insertion of A nucleotide; Allele 2: deletion of GA nucleotides; Biallelic mutant.	Positive	Negative	3 : 1
EC3	Chromatogram from forward sequencing ECWT line GGAACTGGATGGTGATGTGAACGGGCACAAGT EC3 line GGAACTGKWYRSWR----GWWCKSSSWCARSK Allele 1 GGAACTGGATGGTG----GAACGGGCACAAGT **************----**********???? Allele 2 GGAACTG-TT-CACAAGTTCTCCGTCACAA *******--*----*-**---*-*-*****	Allele 1: deletion of ATGT nucleotides; Allele 2: two deletions of G nucleotide; Biallelic mutant.	Positive	Negative	3 : 1
	Chromatogram from reverse sequencing ECWT line GACGGAGAACTTGTGCCCGTTCACATC-ACCA-T-CC EC3 line GACGGAGAACTTGTGMMCRKTY-CA-CMWCSAKTKSS Allele 1 GACGGAGAACTTGTGCCCGTTC-CA-CCACCATTGCC **********************-**-*-****-*-** Allele 2 GACGGAGAACTTGTGAA--CAGTTCACATCGA--GTT-CAGTTCC ***************----*-*********-*----*-*******	Allele 1: deletions of A and T nucleotides and insertions of C/A, T and G nucleotides; Allele 2: insertions of AA, A, G, and G nucleotides and two deletions of CC nucleotides; Biallelic mutant.
EC4	ECWT line GGAACTGGATGGTGATGTGAACGGGCACAAG EC4 line GGAACTGGATGGTGAT-KKARMRSGSRCAMR Allele 1 GGAACTGGATGGTGAT-TGAACGGGCACAAG ****************-************** Allele 2 GGAACTGGATGGTGATGTAGAACGGGCACA ******************-*--*********	Allele 1: deletion of G nucleotide; Allele 2: insertion of A nucleotide; Biallelic mutant.	Positive	Negative	3 : 1
EC5	ECWT line GGAACTGGATGGTGATGTGAACGGGCACAAG EC5 line GGAACTGGATGGTGAT-KRAMCRSSMMCAMK Allele 1 GGAACTGGATGGTGAT-TGAACGGGAACAAG ****************-************** Allele 2 GGAACTGGATGGTGAT--GAACGGGCACA ****************--***********	Allele 1: deletion of G nucleotide; Allele 2: deletions of GT nucleotides; Biallelic mutant.	Positive	Negative	3 : 1
EC6	ECWT line GGAACTGGATGGTGATGTGAACGGGCACAA EC6 line GGAACTGGATGGTGATG--WWSRRSSRSMA Allele 1 GGAACTGGATGGTGATG--AACGGGCACAA *****************--*********** Allele 2 GGAACTGGATGGTGATGTTGAACGGGCA *****************-**********	Allele 1: deletion of TG nucleotides; Allele 2: one insertion of T nucleotide; Biallelic mutant.	Positive	Negative	3 : 1
EC7	ECWT line GGAACTGGATGGTGATGTGAACGGGCAC EC7 line GGAACTGGATGGTGATGWRAMSGGSMMM Allele 1 GGAACTGGATGGTGATGTGAACGGGCAC **************************** Allele 2 GGAACTGGATGGTGATG--AACGGGCACA *****************--***********	Allele 1: no indels; Allele 2: deletion of TG nucleotides; Monoallelic mutant.	Positive	Positive	3 : 1
EC8	ECWT line GGAACTGGATGGTGATGTGAACGGGCACA EC8 line GGAACTGGATGGTGATGKRAMSGGSMMM Allele 1 GGAACTGGATGGTGATG-GAACGGGCACA *****************-*********** Allele 2 GGAACTGGATGGTGATGTGAACGGGCAC ****************************	Allele 1: deletion of T nucleotide; Allele 2: no indels; Monoallelic mutant.	Positive	Positive	3 : 1
EC9	ECWT line GGAACTGGATGGTGATGTGAACGGGCACAC EC9 line GGAACTGGATGGTGATGWRMRSGSRCMM Allele 1 GGAACTGGATGGTGATGTGAACGGGCACAC ****************************** Allele 2 GGAACTGGATGGTGATG--AACGGGCACAC *****************--***********	Allele 1: no indels; Allele 2: deletion of TG nucleotides Monoallelic mutant.	Positive	Positive	3 : 1
EC10	ECWT line GGAACTGGATGGTGATGTGAACGGGCACAC EC10 line GGAACTGGATGGTGATGRAMSGGSACMA Allele 1 GGAACTGGATGGTGATG--AACGGGCACAC *****************--*********** Allele 2 GGAACTGGATGGTGATG-GAACGGGACCAC *****************-************	Allele 1: deletions of TG nucleotides; Allele 2: deletion of T nucleotide; Biallelic mutant.	Positive	Negative	3 : 1