Table 3. Kinetic parameters of GuUGDs.
| Isoform | Kinetics for UDP-glucose | Kinetics for NAD+ | ||||
|---|---|---|---|---|---|---|
| Km (µM) | kcat (s−1) | kcat/Km (s−1 µM−1) | Km (µM) | kcat (s−1) | kcat/Km (s−1 µM−1) | |
| GuUGD1 | 261±84 | 0.09±0.01 | 0.0003 | 31±5 | 0.10±0.01 | 0.0031 |
| GuUGD2 | 285±54 | 0.89±0.09 | 0.0031 | 153±29 | 1.01±0.12 | 0.0066 |
| GuUGD3 | 570±46 | 0.39±0.01 | 0.0007 | 62±7 | 0.40±0.02 | 0.0064 |
| GuUGD4 | 55±5 | 0.25±0.02 | 0.0045 | 122±15 | 0.36±0.01 | 0.0029 |
We calculated enzyme activity based on initial velocity within 5 min measured by monitoring the conversion of NAD+ to NADH, detected by the absorbance at 340 nm. Values represent the mean±SE of three measurements.