Table 1. Relative activity of Cpur5GT against several potential substrates.
Substrate | Product | Relative activity (%) |
---|---|---|
Mv3G | Mv3,5dG | 100a±2.2 |
Dp3G | Dp3,5dG | 86.8±3.3 |
Pt3G | Pt3,5dG | 18.8±2.3 |
Cy3G | Cy3,5dG | 78.3±12.4 |
Pn3G | Pn3,5dG | 72.4±7.1 |
Relative activity analyses were performed with UDP-glucose as the donor. The amount of reaction productwas calculated from the area of the HPLC chromatogram recorded at 525 nm. The activity of Cpur5GTagainst malvidin 3-glucoside (Mv3G) was considered 100%, which was used to determine its relativeactivity against other substrates.