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. 2021 Mar 4;28(8):2333–2350. doi: 10.1038/s41418-021-00755-6

Fig. 1. UUO- induced Casp3/GSDME activation and renal tubular cell necrosis increased in the kidney.

Fig. 1

A Immunostaining images of kidney sections from mice. Kidneys were isolated from mice on day 3 after sham or UUO surgery. Sections were stained using an anti-GSDME antibody (red) and DAPI (blue). n = 6 for Sham group; n = 10 for UUO group. Scale bar = 50 μm. B Freshly isolated tubules were collected for western blot to analyze the cleavage of GSDME and Caspase3. Antibody against GAPDH was used as a loading control. n = 4. C Representative PAS staining of kidney sections from sham-group mice and mice on day 3 or 7 after UUO. Scale bar (black) = 100 μm, scale bar (blue) = 50 μm. D Histologic renal injury scores are shown in panel (C). Scores were obtained by counting the percentage of tubules that displayed tubular necrosis and tubular dilation. ##P < 0.01 vs. sham group. Sham group, n = 6; UUO groups, n = 10. E Representative electron micrographs of a tubular necrotic cell. n = 5. Scale bar = 2.5 μm. F Representative electron micrographs of mitochondrial damage in proximal tubular cells. n = 5. Scale bar = 1 μm. G The activation of caspase 8 and caspase 9 were analyzed in freshly isolated tubules by the western blot at the indicated time. n = 4.