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. 2021 Mar 4;28(8):2333–2350. doi: 10.1038/s41418-021-00755-6

Fig. 3. Bone marrow-derived cells with Gsdme deficiency did not reduce renal tubular damage and renal fibrosis progression after UUO.

Fig. 3

Chimeric mice were created, in which the BM was replaced with donor BM cells from WT or Gsdme-ko. A, E Representative images of PAS-stained obstructive kidney sections (Bar = 100 μM), and quantification of renal injury in BM chimeric mice at 3 days after UUO. n = 9. B, F Representative images of Masson trichrome-stained obstructive kidney sections (Bar = 100 μM), and assessment of renal fibrosis in BM chimeric mice at 13 days after UUO. n = 9. C, D Representative images of immunofluorescence staining with markers (α-SMA and Col-I) of Fibrosis. Scale Bar = 100 μM. G, H Quantification of α-SMA and Col I expression. n = 6. **P < 0.01 vs. WT to WT chimeric mice; ∆∆P < 0.01, vs. KO to WT chimeric mice. I Western blot analysis of protein expression of α-SMA and Col I. n = 4. WT wild type, KO Gsdme-ko.