Table 3.
Maldi-tof analysis of the 9 fractions obtained after HPLC of the digest of Dopa5T-Ctl by the endoprotease Glu-C.
| Fraction | Molecular mass (Da) | Sequence |
|---|---|---|
| 1 | 826.42 | Dopa-K-Dopa-K-Dopa + 1(OX) |
| 2 | 861.11 | Dopa-K-Dopa-K-Dopa + 3(OX) |
| 877.10 | Dopa-K-Dopa-K-Dopa + 4(OX) | |
| 1272.66 | Dopa-K-Dopa-K-Dopa-TLRG + 2(OX) | |
| 1425.78 | Dopa-K-Dopa-K-Dopa-TLRGGE | |
| 1440.77 | Dopa-K-Dopa-K-Dopa-TLRGGE + 1(OX) | |
| 3 | 861.31 | Dopa-K-Dopa-K-Dopa + 3(OX) |
| 877.28 | Dopa-K-Dopa-K-Dopa + 4(OX) | |
| 893.26 | Dopa-K-Dopa-K-Dopa + 5(OX) | |
| 909.21 | Dopa-K-Dopa-K-Dopa + -(OX) | |
| 1069.75 | RSMRLSFR + 1(OX) | |
| 1193.98 | TLRGGERSMR + 2(OX) | |
| 1224.01 | TLRGGERSMR + 4(OX) | |
| 1295.04 | RSMRLSFRAR + 1(OX) | |
| 1426.03 | Dopa-K-Dopa-K-Dopa-TLRGGE | |
| 1449.99 | Dopa-K-Dopa-K-Dopa-TLRGGE + 1(Na+) | |
| 4 | 1068.60 | RSMRLSFR + 1(OX) |
| 5 | 783.51 | RSMRLS + 2(OX) |
| 920.50 | ARGYGFR + 6(OX) | |
| 1129.73 | FRARGYGFR | |
| 6 | 1052.63 | RSMRLSFR |
| 1068.62 | RSMRLSFR + 1(OX) | |
| 7 | 1875.97 | RSMRLSFRARGYGFR + 1(OX) |
| 8 | 1861.36 | RSMRLSFRARGYGFR |
| 1877.25 | RSMRLSFRARGYGFR + 1(OX) | |
| 9 | 1633.07 | MRLSFRARGYGFR + 1(OX) |