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. 2021 Mar 19;28(8):2450–2464. doi: 10.1038/s41418-021-00762-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a MCF-7 and DU4475 cells stably knocking out SNORD50A/B and control cells were lysated, and the lysates were then immunoprecipitated with antibodies to TRIM21 or IgG, and immunoblotted with antibody against GMPS to validate the interaction between GMPS and TRIM21. b Cell lysates of indicated cells were immunoprecipitated with antibody against GMPS, and then immunoblotted with antibody against ubiquitin to evaluate GMPS ubiquitination. c The effect of SNORD50A/B knockdown on inducing cellular colocalization of GMPS and TRIM21 was determined by immunofluorescence assay. Blue color represents DAPI staining for nuclei; Green color represents TRIM21; Red color represents GMPS; Scale bars, 10 µm. d MCF-7 and DU4475 cells ectopically expressing SNORD50A/B and control cells were lysated, and the lysates then were immunoprecipitated with antibody to TRIM21 or IgG, and immunoblotted with antibody to GMPS to determine the interaction between GMPS and TRIM21. e The effect of restoring SNORD50A/B expression in SNORD50A/B-deleted MCF-7 and DU4475 cells on the interaction between GMPS and TRIM21.