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. 2021 Aug 2;11:15648. doi: 10.1038/s41598-021-95157-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of several innate immune pathways is impaired in MB49 cells. MB49 tumor cells and bone marrow-derived macrophages (BMDMs) were stimulated with Pam3CSK4 (1 µg/ml), ultrapure LPS (100 ng/ml), CpG-ODN (1 µg/ml) or BCG (MOI 10, 20 or 40). MB49 and BMDM cells were transfected with PolyI:C (3 µg/ml), dsDNA90 (3 µg/ml) or cGAMP (6 µg/ml) in addition to Lipofectamine 2000 (3ug/ml). The production of TNF-α (A), IL-6 (B) and CXCL10 (C) in cell supernatants was analyzed after 24 h of stimulus by ELISA. IFN-β expression after 1 h was determined by qPCR (D). All qPCR results were relative to β-actin mRNA as a normalizer. MB49 unstimulated sample (Mock) or BMDM (Mock) were used as control. The values are representative of at least two independent experiments. *Statistically significant compared to mock from the same cell line, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.