Figure 4.

The effect of BCG treatment on subcutaneous tumor is independent of cGAS-STING and TLR3 signaling pathways. C57BL/6 WT, STING^−/−, cGAS^−/− and TLR3^−/− mice were submitted to the subcutaneous tumor model injection and BCG treatment. Tumor growth curves are shown for STING^−/− (A), cGAS^−/− (B) and TLR3^−/− (D). The final volumes (day 22) comparing C57BL/6 WT with STING^−/− and cGAS^−/− animals are shown in Figure (C) and C57BL/6 WT compared to TLR3^−/− in Figure (E). Data represents mean and standard deviation from the results of at least two independent experiments. C57BL/6 WT data represents the summary of control animals from all in vivo experiments comparing WT and KO mice. *Statistically significant compared to the respective untreated control, ****P ≤ 0.0001.