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. 2021 Jul 20;12:693839. doi: 10.3389/fmicb.2021.693839

TABLE 1.

Composition of experimental diets (as-fed basis).

Ingredientsa,% Pre-care period Late-care period
0.00 0.20 0.40 0.60 0.80 0.00 0.20 0.40 0.60 0.80
Waxy corn starch 54.80 45.21 38.36 33.43 29.32 53.54 44.17 37.48 32.66 28.64
Hi-maize 1,043 9.59 16.44 21.37 25.48 9.37 16.06 20.88 24.90
Soybean meal 9.00 9.00 9.00 9.00 9.00 12.00 12.00 12.00 12.00 12.00
Full-fat extruded soybean 9.00 9.00 9.00 9.00 9.00 10.00 10.00 10.00 10.00 10.00
Fermented soybean meal 8.00 8.00 8.00 8.00 8.00 10.00 10.00 10.00 10.00 10.00
Fish meal 5.00 5.00 5.00 5.00 5.00 5.80 5.80 5.80 5.80 5.80
Whey, dried 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00
Plasma protein powder 4.84 4.84 4.84 4.84 4.84
Monocalcium phosphate 1.17 1.17 1.17 1.17 1.17 1.33 1.33 1.33 1.33 1.33
Soybean oil 1.00 1.00 1.00 1.00 1.00
Premixb 0.92 0.92 0.92 0.92 0.92 0.92 0.92 0.92 0.92 0.92
Choline chloride 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08
Limestone 0.74 0.74 0.74 0.74 0.74 0.72 0.72 0.72 0.72 0.72
DL-Lysine 0.23 0.23 0.23 0.23 0.23 0.21 0.21 0.21 0.21 0.21
DL-Methionine 0.22 0.22 0.22 0.22 0.22 0.21 0.21 0.21 0.21 0.21
Salt 0.19 0.19 0.19 0.19 0.19
Total 100 100 100 100 100 100 100 100 100 100
Calculated nutrient contentc
Amylose/amylopectin ratiod 0.00 0.20 0.40 0.60 0.80 0.00 0.20 0.40 0.60 0.80
Digestive energy, kcal/kg 3,500 3,500 3,500 3,500 3,500 3,408 3,408 3,408 3,408 3,408
Crude protein,% 18.50 18.50 18.50 18.50 18.50 18.00 18.00 18.00 18.00 18.00
Ca,% 0.85 0.85 0.85 0.85 0.85 0.92 0.92 0.92 0.92 0.92
Av.P,% 0.42 0.42 0.42 0.42 0.42 0.48 0.48 0.48 0.48 0.48
Salt,% 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50
Amino acids,%SIDe
Lys 1.45 1.45 1.45 1.45 1.45 1.31 1.31 1.31 1.31 1.31
TSAA 0.79 0.79 0.79 0.79 0.79 0.71 0.71 0.71 0.71 0.71
Thr 0.83 0.83 0.83 0.83 0.83 0.74 0.74 0.74 0.74 0.74
Trp 0.25 0.25 0.25 0.25 0.25 0.23 0.23 0.23 0.23 0.23

aDAR of waxy corn starch and Hi-maize 1,043 were 0.00 and 1.00, respectively.

bVitamin–mineral premix supplied per kilogram of feed: 10,000 IU of vitamin A, 1,000 IU of vitamin D3, 80 IU of vitamin E, 2.0 mg of vitamin K3, 0.03 mg of vitamin B12, 12 mg of riboflavin, 40 mg of niacin, 25 mg of D-pantothenic acid, 0.25 mg of biotin, 1.6 mg of folic acid, 3.0 mg of thiamine, 2.25 mg of pyridoxine, 300 mg of choline chloride, 150 mg of Fe (FeSO4), 100 mg of Zn (ZnSO4), 30 mg of Mn (MnSO4), 25 mg of Cu (CuSO4), 0.5 mg of I (KIO3), 0.3 mg of Co (CoSO4), 0.3 mg of Se (Na2SeO3), and 4.0 mg of ethoxyquin.

cNutrient content of diets based on estimated nutrient contents of ingredients according to NRC (2012).

dAnalyzed data. Amylose and amylopectin contents were determined by using their assay kits (l-AMYL, Megazyme International Ireland Ltd., Wicklow, Ireland).

eSID, standardized ileal digestible.