TABLE 7.

Effect of DAR on gene expression of intestinal mucosa of weaned piglets challenged with E. coli LPS.

Items1	0.00		0.20		0.40		0.60		0.80		SEM	P-value
	LPS	SAL	LPS	SAL	LPS	SAL	LPS	SAL	LPS	SAL		DAR	STRESS	D*S
Jejunal mucosa
Claudin-1	0.75B	1.04	0.98B	1.03	1.01B	1.02	1.11A	1.51	0.79B	1.16	0.04	0.045	0.01	0.49
ZO-1	0.92	1.03	0.96	1.04	0.96	1.00	0.82	0.71	0.61	0.96	0.04	0.15	0.22	0.46
IL-1β	0.84	1.11	0.60	0.91	0.39	0.73	0.65	0.77	0.37	0.87	0.08	0.53	0.06	0.97
TNFα	1.25	1.06	0.75	1.63	0.61	0.83	0.65	0.73	0.47	0.79	0.13	0.50	0.32	0.78
Ileal mucosa											0.00
Occludin-1	1.49	1.06	1.27	1.93	1.08	1.44	0.98	1.05	1.08	1.62	0.10	0.55	0.26	0.51
Claudin-1	1.15Bb	1.04	2.37Aa	2.20	1.25Bb	1.53	1.13Bb	1.24	1.22Bb	1.38	0.05	< 0.0001	0.60	0.60
ZO-1	1.07	1.06	1.07	1.25	1.21	1.02	0.82	0.92	0.90	1.11	0.06	0.63	0.65	0.84
IL-1β	0.80	1.14	1.12	1.60	0.79	1.16	0.62	2.45	1.00	1.16	0.18	0.82	0.09	0.60
TNFα	0.76Cb	1.02	0.97BCab	1.02	1.96Aa	1.25	1.30Aa	1.84	1.46ABab	1.42	0.07	0.01	0.87	0.10

¹LPS, lipopolysaccharide; SAL, saline; DAR, dietary amylose/amylopectin ratio.

Means within each row without the same superscript letter (A, B, and C) significantly differ (P < 0.05) and (a, b, and c) highly significantly differ (P < 0.01).

ZO-1, tight junction protein zonula occluden-1; IL-1β, interleukin 1β; TNFα, tumor necrosis factor alpha.