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. 2021 Jul 20;15:685715. doi: 10.3389/fnins.2021.685715

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Copyright © 2021 Fang, Wang, Zhou, Shao, Sun, Liang, He, Jiang, Liu, Jin, Fang and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electroacupuncture (EA) regulates the transition from acute to chronic pain induced by ψεRACK injection. Mechanical (A) and thermal (B) responses of Car/ψεRACK-injected animals to EA administration. n = 6. (C) PKCε and TRPV1 staining in the peripheral nervous system 48 h after ψεRACK injection. Scale bar, 100 μm. The quantification of the PKCε–IR positive (D) and TRPV1–IR positive (E) neurons. The quantification of the Western blot results and a representative Western blot showing PKCε (F) and TRPV1 (G) protein isolated from the DRG 48 h after ψεRACK injection. *, **Compared with the NS+PGE2 group, P < 0.05, P < 0.01; ^△△ compared with the ψεRACK group, P < 0.01.